Application of Combined Mixture Process Design for Enhancement of Methane Production Using Co-digestion of Chicken Manure and Napier Grass


Supawadee Yodthongdee ,Panomchai Weerayutsil,Kulyakorn Khuanmar,



Biogas,Methane Gas,Co-Digestion ,Chicken Manure,Napier Pakchong ,Combined Mixture Process Design,


This research is aimed at application of Combined Mixture Process Design by Design Expert program in order to enhance the efficiency of methane production by co-digestion of chicken manure and Napier Pakchong 1 grass (Pennisetum purpureum. Schum), which enables deduction of operation time and cost for methane production. In addition, the impact of co-digestion in terms of C/N ratio was studied. The experimental result indicated that the Combined Mixture Process Design by biogas and methane yield as response variables were significantly appropriate based on the R2 at 93.99% and 93.67%, respectively. It also indicated the factors that enhance the maximum production of methane, i.e., the ratio of inoculum: chicken manure: Napier grass of 59.70: 6.02: 34.28%TS at the total solids of 2.5% of the working volume, pH 8, and 46°C. Such optimum conditions could yield accumulated biogas and accumulated methane of 920.88 ml/gVS and 492 mlCH4/gVS or 73.19%. Comparing to the individual digestion and the co-digestion, it was found that methane production presented the higher methane yield from the co-digestion of chicken manure and Napier grass.


I.Álvarez, J.A., Otero, L., Lema, J.M.(2010). A methodology for optimising feed composition for anaerobic co-digestion of agro-industrial wastes. Bioresour. Technol. 101(4): 1153-1158.

II.APHA and WEF. (2005). Standard methods for the examination of water and wastewater. 21: 258–259.

III.AOAC. Official Methods of Analysis. 12th ed.(1995)Association of Official Analytical Chemists, Washington, DC.

IV.Forster-Carneiro, T., Perez,M., Romero, L.I., (2007). Composting potential of different inoculum sources in the modified SEBAC system treatment of municipal solid wastes. Bioresour. Technol. 98: 3354–3366.

V.Hartman, H., Ahring, B.K. (2005). Anaerobic digestion of the organic fraction of municipal solid waste: Influence of co-digestion with manure. Water Research. 39(8):1543-1552.

VI.Habiba, L., Hassib, B., Moktar, H. (2009). Improvement of activated sludge stabilisation and filterability during anaerobic digestion by fruit and vegetable waste addition. Bioresour. Technol. 100, 1555–1560.

VII.Kaparaju, P., Rintala, J. (2011). Mitigation of greenhouse gas emissions by adopting anaerobic digestion technology on dairy, sow and pig farms in Finland. Renewable Energy 36(1):31–41.

VIII.Li, C., Stromberg, S., Liu, G., Nges, L.A, Liu, N. (2017). Assessment of regional biomass as co-substrate in the anaerobic digestion of chicken manure: Impact of co-digestion with chicken processing waste, seagrass and Miscanthus, Bioresour. Technol. 118:1-10.

IX.N. Subramonia Pillai, P. Seeni Kannan, S.C. Vettivel, S. Suresh. (2017). Optimization of transesterification of biodiesel using green catalyst derived from Albizia Lebbeck Pods by mixture design. Renewable Energy. 104:185-196.

X.Wang, X., Yang, G., Feng, Y.,Ren, G., Han, X. (2012). Optimizing feeding composition and carbon-nitrogen ratios for improved methane yield during anaerobic codigestion of dairy, chicken manure and wheat straw. Bioresour. Technol. 120:78–83.

XI.Wang, X., Yang, G., Li, F., Feng, Y., Ren,G., Han, X. (2013). Evaluation of two statistical methods for optimizing the feeding composition in anaerobic co-digestion: Mixture design and central composite design. Bioresour. Technol. 131:172-178.

XII.Wilawan, W., Pholchan, P. and Aggarangsi, P.(2014). Biogas production from co-digestion of Pennisetum purureum cv. Pakchong 1 Grass and layer chicken manure using completely stirred tank. Energy Procedia, 52:216-222.

XIII.Sosnowski, P.,Wieczorek, A., Ledakowicz, S. (2013). Anaerobic co-digestion of sewage sludge and organic fraction of municipal solid wastes. Advances in Environmental Research. 7(3): 609-616.

XIV.Mshandete, A., Kivaisi, A., Rubindamayugi, M., Mattiasson, B. (2004). Anaerobic batchco-digestion of sisal pulp and fish wastes. Bioresour. Technol.95(1):19–24.

View | Download